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OBJECTIVE:To study the effects of maggot oil on the healing of rat with acute skin trauma and infection and its mechanism,in order and to provide reference for further development of maggot oil. METHODS:SD rats were randomly divided into normal group,model group,maggot oil group and Jingwanhong treatment group (positive control),with 70 mice in each group. Except for normal group,acute skin trauma and infection model was induced in other groups by smearing Staphylococus aureus suspension at the wound. After modeling,normal group and model group were given normal saline,and maggot oil group and Jingwanhong treatment group were given relevant medicine 0.3 mL/100 g,at 9:00 and 17:00,for consecutive 15 d. Wound and wound healing time of rats were observed in each group. The content of hydroxyproline in wound was determined in 10 rats of each group after 1,2,4,6,8 d of administration. The content of water in wound was determined after 2,4,6,8 d after administration. 2 h after last administration,the content of lysozyme,the levels of inflammatory factors (TNF-α,IL-6),the expression of NF-κB p65(in cytoplasm and nucleus)and p-IκB-α(in cytoplasm)protein were determined in 10 rats of each group. RESULTS:Compared with normal group,wound edema of model group was obvious,and wound healing time was(14.3±2.1)d. After 4,6,8 d of medication,the content of hydroxyproline in wound of rats was decreased significantly in model group (P<0.05 or P<0.01). After 2,4,6,8 d of medication,the content of water in wound was increased significantly in model group(P<0.01). After 15 d of medication,the serum contents of lysozyme,TNF-α and IL-6 in rats were increased significantly in model group (P<0.01). The expression of NF-κB p65 (in cytoplasm) in wound was decreased significantly (P<0.01),while the expressions of NF-κB p65 (in nucleus) and p-IκB-α(in nucleus) protein were increased significantly (P<0.01). Compared with model group,above indicators of administration groups were improved significantly (P<0.05 or P<0.01). CONCLUSIONS:Maggot oil could protect tissue injury induced by acute skin wound infection,promote wound healing. The possible mechanism might play anti-inflammatory effect through promoting collagen production,increasing lysozyme content,regulating NF-κB signal pathway.
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Objective To investigate the technical requirements for the 3.0T multi-modal quantitative MR imaging of the rabbit liver.Methods A total of 8 rabbits were scanned and T1 mapping,T2 mapping,MT and Gd EOB-DTPA dynamic enhancement images were acquired.For the dynamic enhanced scanning,injecting contrast medium was followed by saline flush with different combinations of injection flow rate (1.5 mL/s and 2 mL/s) and injection volume(6 mL and 8 mL).The quality of the images was assessed and analyzed statistically.Results The total scanning time was about 20-25 min.High-quality images were acquired by T1 mapping,T2 mapping and MT sequences.There was no significant difference in image quality among different groups in Gd-EOB-DTPA dynamic enhanced scanning(P＞0.05).Two rabbits died when the combination of injection flow rate of 2 mL/s and injection volume of 8 mL was used.Conclusion 3.0 T multi-modal quantitative MR scanning of rabbit liver can be achieved successfully if scanning parameters are properly chosen.
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Objective:To investigate the feasibility of targeted imaging and therapy of prostate cancer using nanocomposite probes composed of fluorescent magnetic nanoparticles(FMCNPs) and single chain Fv(ScFv) antibody specific for gama-seminoprotein.Methods:The nanocomposite probes(FMCNPs-ScFv) were prepared by conjugating fluorescent magnetic nanoparticles with singlegama-chain Fv antibody specific gama-seminoprotein,and were characterized by high resolution transmission electron microscopy,fluorescent spectrum and magnetic spectrum.Nanocomposite probes were incubated with prostate cancer LNCaP cells,and the targeting results of nanocomposite probes were observed by fluorescent microscopy.The cytotoxicity effect of the nanocomposite probes was measured by MTT.Nude mice models of prostate cancer were established and identified by immunohistochemistry method.The nanocomposite probes were injected into nude mice via tail vein.The distribution of nanocomposite probes in the nude mice was observed by Micro-animal imaging system,targeted imaging of the prostate cancer was observed by MR instrument.The nude mice with prostate cancer were irradiated with 100 W magnetic field for 30 min,and the changes of tumor sizes were observed.Results:The FMCNPs-ScFv nanocomposite probes were successfully prepared.Nanocomposite probes entered into the cytoplasm of cancer cells and exhibited low cytotoxicity effect.Nude mice model with prostate cancer were successfully fabricated;the nanocomposite probes distributed quickly in the main organs of mice,and gradually concentrated on the tumor tissues within 24 h.MR images showed that the tumor images were gradually enhanced from 6 h to 24 h after injection of the nanocomposite probe.Four days after magnetic irradiation,the tumors in the nude mice grew slower compared with the control nude mice(P
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Objective To analyse the MRI appearances of giant cell tumors of the bone and the relationship with their pathologic bases.Methods Histologic examinations were conducted in 21 cases,and their correlations with the MRI appearances were further evaluated.Results There were 3 cases of the Ⅰ grade,3 cases of the Ⅰ～Ⅱ grade,13 cases of the Ⅱ grade,2 cases of the Ⅲ grade of the giant cell tumors.Most tumors were composed of solid content that presented sligthly low to intermediate signal intensity on T 1WI,sligthly high to high signal intensity on T 2WI.The minority of the tumors were composed of the half of the solid and cystic contents expectively.Specific tumors was mainly composed of cystic content or combined with fluid-fluid level on T 2WI.Soft tissue masses were found in 8 cases.Conclusion The location and their extension into the soft tissue of the tumors can be clearly demonstrated on MR imaging,but there is no relationship between the grades of the tumors and their MRI appearances.
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Objective To evaluate the value of MR in the diagnosis of glomus jugulare tumors.Methods 15 cases (8 in the right and 7 in the left-side) of glomus jugulare tumors were retrospectively analysed, with the emphasis on the locations, MR signal intensities andthe invading appearances to the surrounding structures of tumors. Results The tumors demonstrated as oval or irregular shape. Thesurrounding bones around the jugular fossa were destroyed in 12 masses. The signal intensity of the tumors was heterogeneous and with marked “salt and pepper”appearance in 12 masses(12/15) on plain MR imaging. The masses(13/13)were all markedly enhanced after the injection of Gd-DTPA, in which 10 of them presented with “salt and pepper”appearance. MR angiography was performed in 9 cases, theinternal carotid artery and the internal jugular vein were all displaced and/or encompassed.Conclusion Glomus jugulare tumor can becorrectly diagnosed according to the combination of MR imaging features with the typical localization,“salt and pepper” appearance and bone erosion in the skull base.
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Objective To analyze the MR findings of vagal paraganglioma.Methods Six cases of vagal paraganglioma(4 benign and 2 malignant)were retrospectively analyzed with the emphasis on the location,MR signal intensity,dislocation of the carotid vessels,and the metastasis of the tumor.Results The tumors located in the superior(4 cases),middle(1 case)and inferior(1 case)neck,respectively,with right-sided in 4 cases and left-sided in 2 cases.The signal intensities of the six tumors were heterogeneous,combined with 'salt and pepper' appearance in 4 cases in which the 'pepper' appearance was more marked.On MR angiography performed in 4 cases,carotid arteries were found dislocated anteriomedially.Two cases were diagnosed as malignancies by the manifestations of destruction of surrounding bone,metastasis of bilateral lungs and/or the lymph nodes.Conclusion Vagal paragangliomas could be correctly diagnosed before surgery according to their locations and signal intensities.
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Objective To observe the clinical effect of Yangjinhua Powder Capsule(Flos Daturae Powder Capsule)in the treatment of Parkinson's disease(PD).Methods Of the 51 PD patients,15 who came for the first visit were administered Yangjinhua Powder Capsule only,and 36 who had been taking western medicine were given Yangjinhua Powder Capsule without stopping using the original medicine.The Unified Parkinson Disease Rating Scale(UPDRS)was used to score the patients before treatment,7 days,3 months,and 6 months after treatment respectively,and as well to evaluate the total effect and untoward reactions.Results After treatment,29(56.86%)patients were remarkably improved;11(21.57%)patients were improved;and 2(3.92%)patients were slightly improved.Totally 42 patients were improved.The total effective rate was 82.35%.After 7 days' treatment,the improvement of tremor was significant(P
